Pair correlation for images (2D maps and connectivity maps)
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چکیده
Start SimFCS, in the RICS page, first load a file to be analyzed. Generally, this file consists of a stack of images either acquire with the TIRF or SPIM microscope. You should have on the order of 5000 to 10,000 frames or more. In the example below I used a simulation of a region 64x64 pixels with about 1000 molecules. The pixel size is 50nm so that the total field of view is 3.2um and the PSF is 200nm. The density is 0.01 molecules per um. The diffusion coefficient was 10um/s and the camera frame rate was about 62.5 frames/s. Some simulations were done with slightly different parameters. The total number of frames was 8192 or higher.
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